Three-photon excitation in fluorescence microscopy.

نویسندگان

  • S W Hell
  • K Bahlmann
  • M Schrader
  • A Soini
  • H M Malak
  • I Gryczynski
  • J R Lakowicz
چکیده

We show experiments proving the feasibility of scanning fluorescence microscopy by three-photon excitation. Three-photon excitation fluorescence axial images are shown of polystyrene beads stained with the fluorophore 2,5-bis(4-biphenyl)oxazole (BBO). Three-photon excitation is performed at an excitation wavelength of 900 nm and with pulses of 130 fs duration provided by a mode-locked titanium-sapphire laser. Fluorescence is collected between 350 and 450 nm. The fluorescence image signal features a third-order dependence on the excitation power, also providing intrinsic 3-D imaging. The resolution of a three-photon excitation microscope is increased over that of a comparable two-photon excitation microscope.

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عنوان ژورنال:
  • Journal of biomedical optics

دوره 1 1  شماره 

صفحات  -

تاریخ انتشار 1996